Figure 2.

LUM deletion alleviated TNF-α-induced cell cycle arrest and cellular senescence of hNPCs. Analysis of cell viability (a), cell cycle distribution (b-c) and the expression of cell cycle markers (d) by use of CCK-8, flow cytometry and western blot in hNPCs stimulated with TNF-α or transfected with si-NC/si-LUM-1. Analysis of senescent cells (e), telomerase activity (f) and the expression of senescence markers (g) by means of β-galactosidase staining, telomerase ELISA and western blot in hNPCs stimulated with TNF-α or transfected with si-NC/si-LUM-1. N = 3. ***P < 0.001 vs. control; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. TNF-α+ si-NC