Figure 3.

LUM silencing downregulated ASK1/P38 signaling pathway through FasL in TNF-α-induced hNPCs. (a) Detection of FasL expression level by western blot in hNPCs stimulated with TNF-α or transfected with si-NC/si-LUM-1. N = 3. ***P < 0.001 vs. control; ^###P < 0.001 vs. TNF-α+ si-NC. (b–c) Detection of the overexpression efficacy of Oe-FasL by means of RT-qPCR and western blot assay. N = 3. ***P < 0.001 vs. Oe-NC. (d) The expression of phosphorylated ASK1 and p38 detected by western blot in TNF-α-stimulated hNPCs transfected with si-NC/si-LUM-1 or co-transfected with si-LUM-1 and Oe-NC/FasL. N = 3. ***P < 0.001 vs. control; ^###P < 0.001 vs. TNF-α+ si-NC; ^ΔΔΔP<0.001 vs. TNF-α+ si-LUM-1+ Oe-NC