Figure 4.

LUM silencing alleviated TNF-α-induced inflammatory response and cell cycle arrest of hNPCs by inhibiting ASK1/p38 signaling pathway via inactivating FasL expression. (a–c) The contents of pro-inflammatory cytokine was detected by ELISA in TNF-α-stimulated hNPCs transfected with si-NC/si-LUM-1 or co-transfected with si-LUM-1 and Oe-NC/FasL. Analysis of cell viability (d), cell cycle distribution (e–f) and the expression of cell cycle markers (g) by use of CCK-8, flow cytometry and western blot in TNF-α-stimulated hNPCs transfected with si-NC/si-LUM-1 or co-transfected with si-LUM-1 and Oe-NC/FasL. N = 3. **P < 0.01, ***P < 0.001 vs. TNF-α+ si-NC; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. TNF-α+ si-LUM-1+ Oe-NC; ^ΔΔΔP<0.001 vs. TNF-α+ si-LUM-1+ Oe-FasL