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. 2021 Oct 4;12(1):7631–7643. doi: 10.1080/21655979.2021.1981798

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — CircPRKDC facilitates GC progression via the miR-493-5p/IRS2 axis. (a) RT-qPCR showed the relative expression of IRS2 in HGC27 and AGS cells transfected with shNC, shcircPRKDC, shcircPRKDC+pcDNA3.1/IRS2. (b and c) CCK-8 and colony formation assays showed the viability of HGC27 and AGS cells transfected with shNC, shcircPRKDC, shcircPRKDC+pcDNA3.1/IRS2. (d and e) Transwell assay showed that the migration and invasion abilities of HGC27 and AGS cells transfected with shNC, shcircPRKDC, shcircPRKDC+pcDNA3.1/IRS2. (f) Western blot showed the protein levels of E-cadherin and N-cadherin in HGC27 and AGS cells transfected with shNC, shcircPRKDC, shcircPRKDC+pcDNA3.1/IRS2. *p < 0.05, **p < 0.01, ***p < 0.001