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miR-580-3p inhibitor or TWIST1 overexpression reversed effects of sh-circRAB3IP. MG63 cells were transfected with the control sh RNA (sh-NC), sh-circRAB3IP, sh-circRAB3IP+miR-580-3p inhibitor or sh-circRAB3IP+TWIST1 overexpression vector (sh-circRAB3IP+oeTWIST1). (a, b) Cell proliferation was detected by CCK-8 assay and colony formation assay. (c, d) Transwell assay was employed to detect cell migration and invasion of OS cells. *P < 0.05
