Figure 9.

Effects of rhein on apoptosis and ER stress of adipose tissue in SD rats fed with HFD. The rats of different groups received the following treatment for six weeks, respectively. normal chow, high-fat diet (HFD) (D12492, 60% kcal%, Research Diets, USA), high-fat diet +Rhein-L (lose dose), high-fat diet (60% kcal%) + Rhein-M (medium dose), high-fat diet (60% kcal%) +Rhein-H (high dose). (a). Tunel staining for adipose tissue. (b). western blot analysis of adipose tissue showing the expression of caspase12, caspase3 and cleaved caspase3. n = 6 rats/group. HFD, high-fat diet-fed rats. ***p < 0.05 versus control. #p < 0.05, ##p < 0.01, ###p < 0.001 versus HFD