Figure 11.

Rhein treatment decreased lipid formation and apoptosis of 3T3-L1 induced by inflammatory factors. The supernatant culturing Raw246.7 cells with LPS stimulation was collected and used to incubate with 3T3-L1 preadipocytes. (a). The levels of inflammatory factors, IL-6, IL-1β and TNF-α, in LPS-induced Raw246.7 macrophages. (b). Quantitative analysis of lipid droplet accumulation. (c, d). Detection of cell apoptosis by flow cytometry in 3T3-L1 cells. (e). Representative images of caspase12, caspase3 and cleaved caspase3 expression by Western blot. Statistical analysis was performed using one-way ANOVA followed by Tukey’s multiple comparison test. Each experiment was repeated at least three times. ***p < 0.001 versus control, #p < 0.05, ##p < 0.01, ###p < 0.001 versus Model