Table 1.
Gene | Primer sequence (5’-3’) |
---|---|
EGR1 | F: CCTATGAGCACCTGACCACA |
R: ATCGTTTGGCTGGGATAACTC | |
miR-675 | F: TGGTGCGGAGAGGGC |
R: GAACATGTCTGCGTATCTC | |
SESN3 | F: CCGCCAGTAACTATCATACATGCG |
R: GAGGATGTTGACACAACCAT | |
GAPDH | F: CGGAGTCAACGGATTTGGTCGTAT |
R: AGCCTTCTCCATGGTGGTGAAGAC | |
U6 | F: CGCAAGGATGACACGCAAAT |
R: ATTTGCGTGTCATCCTTGCG |
Note: RT-qPCR, reverse transcription quantitative polymerase chain reaction; miR-675, microRNA-675; SESN3, sestrin 3; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F: forward; R, reverse