Figure 3.

Circ_0074673 serves as an miR-1200 sponge. (a) MiR-1200 targets the 3′-UTR sequence of circ_004673. (b) The relative luciferase activity was determined using the luciferase reporter assay. (c) Circ_0074673 and miR-1200 co-immunoprecipitated with AGO2 was determined by RIP assay. (d) Circ_0074673 pulled-down with biotin-labeled miR-1200 by RNA pull-down assay. (e) Following si-circ_0074673 transfection, the levels of miR-1200 were detected using qPCR. Each experiment was repeated in three times. **P < 0.01. ns P > 0.05