Figure 5.

MiR-1200 directly targets MEOX2. (a) MiR-1200 targets the 3′-UTR region of MEOX2. (b) The relative luciferase activity was measured using the luciferase reporter assay. (c) MiR-1200 and MEOX2 co-immunoprecipitated with AGO2 was determined by RIP assay. (d) MEOX2 pulled-down with biotin-labeled miR-1200 using RNA pull-down assay. (e) The levels of MEOX2 following transfection with miR-1200 mimics were detected using qPCR. Each experiment was repeated in three times. **P < 0.01. ns P > 0.05