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. 2021 Sep 14;12(1):6713–6723. doi: 10.1080/21655979.2021.1971484

Figure 3.

Figure 3.

miR-153-3p is a target of FGD5-AS1 in DDP-resistant HCC cells. (a) StarBase database was used to predict the binding site between FGD5-AS1 and miR-153-3p. Dual luciferase reporter assay was used to determine the relative luciferase activity of FGD5-AS1-WT or FGD5-AS1-Mut vectors in (b) Huh7/DDP and (c) Hep3B/DDP cells co-transfected with NC mimic or miR-153-3p mimic. (d) RIP assay was used to measure the enrichment level of FGD5-AS1 in Huh7/DDP and Hep3B/DDP cells transfected with miR-153-3p mimic or NC mimic. (e) RT-qPCR was used to analyze the relative expression levels of miR-153-3p in DDP-resistant and -sensitive HCC cells. (f) RT-qPCR was used to determine relative miR-153-3p expression levels in Huh7/DDP and Hep3B/DDP cells transfected with shNC, shFGD5-AS1, pcDNA3.1 and pcDNA3.1/FGD5-AS1. *P < 0.05