Figure 5.

TWF1 is a target of miR-153-3p. (a) StarBase database was used to predict the binding site between TWF1 and miR-153-3p. Dual luciferase reporter assay was used to determine the relative luciferase activity of TWF1-WT or TWF1-Mut in (b) Huh7/DDP and (c) Hep3B/DDP cells co-transfected with NC mimic or miR-153-3p mimic. (d) RT-qPCR was used to analyze relative TWF1 expression in DDP-resistant HCC tissues (n = 32) and DDP-sensitive HCC tissues (n = 28). (e) RT-qPCR and (f) western blotting were used to determine the relative mRNA and protein expression of TWF1, respectively, in Huh7/DDP and Hep3B/DDP cells. (g) RT-qPCR and (h) western blotting were used to determine the relative mRNA and protein expression of TWF1 in Huh7/DDP and Hep3B/DDP cells transfected with NC mimic, miR-153-3p mimic, NC inhibitor and miR-153-3p inhibitor. *P < 0.05