Figure 6.

TWF1 knockdown decreases DDP resistance in DDP-resistant hepatocellular carcinoma cells by modulating miR-153-3p expression. (a) RT-qPCR was used to determine relative miR-153-3p expression in DDP-resistant cells transfected with NC inhibitor and miR-153-3p inhibitor. (b) RT-qPCR was used to determine relative TWF1 expression in Huh7/DDP and Hep3b/DDP cells transfected with shNC, shTWF1 or shTWF1 + miR-153-3p inhibitor. (c) CCK-8 assay was used to determine the IC₅₀ value of DDP in DDP-resistant cells transfected with shNC, shTWF1 and shTWF1 + miR-153-3p inhibitor. (d and e) CCK-8, (f) colony formation and (g) Transwell assays were used to examine the proliferation and invasion, respectively, of Huh7/DDP and Hep3B/DDP cells transfected with shNC, shTWF1 and shTWF1 + miR-153-3p inhibitor. (h) TUNEL assays were used to assess the apoptosis of Huh7/DDP and Hep3B/DDP cells transfected with shNC, shTWF1 and shTWF1 + miR-153-3p inhibitor. (i) RT-qPCR and (j) western blotting were used to determine relative TWF1 expression in Huh7/DDP and Hep3B/DDP cells transfected with shNC, shFGD5-AS1 and shFGD5-AS1 + miR-153-3p inhibitor. *P < 0.05