Figure 3.

Circ_0008274 adsorbs miR-140-3p

(a).The potential binding site between circ_0008274 and miR-140-3p was predicted by Circinteractome database.(b). The effects of miR-140-3p mimics or miR-140-3p inhibitors on the relative luciferase activity of the reporters carrying the sequence of circ_0008274, were detected by dual-luciferase reporter gene assay.(c). RIP assay was used to detect the enrichment degree of circ_0008274 and miR-140-3p, to indicate their interaction.(d). The expression of miR-140-3p in HCC cells was detected by qRT-PCR after overexpression or knockdown of circ_0008274.(e).The expression of miR-140-3p in HCC tissues and adjacent tissues was detected by qRT-PCR. (f). The expression of miR-140-3p in HCC cell lines and THLE-3 cells was detected by qRT-PCR. (g). Pearson correlation analysis showed the correlation between circ_0008274 and miR-140-3p expressions in HCC tissues.* P < 0.05, ** P < 0.01, and *** P < 0.001.