Figure 5.

Circ_0008274 regulates miR-140-3p/GRN axis

A.The potential binding sites between GRN mRNA 3’-UTR and miR-140-3p were predicted by TargetScan database.B.The relative luciferase activity was detected by dual-luciferase reporter gene assay.C&D. The effects of circ_0008274 and miR-140-3p on the expression of GRN protein and mRNA were detected by western blot and qRT-PCR.E. The expression of GRN mRNA in HCC tissues and adjacent tissues was detected by qRT-PCR.F&G. Pearson correlation analysis was used to detect correlations between miR-140-3p expression and GRN mRNA expression, and miR-140-3p expression and GRN mRNA expression in HCC samples.* P < 0.05, ** P < 0.01, and *** P < 0.001.