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. 2021 Sep 13;12(1):6083–6095. doi: 10.1080/21655979.2021.1971503

Figure 3.

Figure 3.

PCED1B-AS1 serves as a ceRNA by sponging miR-215-3p in GC

A. The expression of PCED1B-AS1 in the nuclear fraction and cytoplasmic fraction of GC cells was detected by qRT-PCR. U6 and GAPDH were detected in nuclear and cytoplasmic fractions.B. Potential binding sites between PCED1B-AS1 and miR-215-3p were analyzed through the LncBase Predict v.2 database.C. RIP assay was used to validate the interaction between PCED1B-AS1 and miR-215-3p.D. The targeting relationship between PCED1B-AS1 and miR-215-3p was confirmed by dual-luciferase reporter gene assay.E. qRT-PCR was used to detect the expression of miR-215-3p in 48 cases of GC tissues and normal adjacent tissues.F. The expression of miR-215-3p in GES-1 cells and GC cell lines (HGC-27, KATO III, NCI-N87 and AGS) was detected by qRT-PCR.G. Spearman’s correlation analysis was used to analyze the correlation between PCED1B-AS1 expression and miR-215-3p expression in tissues.H. qRT-PCR was used to detect the expression of miR-215-3p in GC cells, after PCED1B-AS1 was overexpressed or knocked down.*P < 0.05, ** P < 0.01, and *** P < 0.001.