Figure 4.

CXCR1 is a target mRNA of miR-215-3p

A. The potential binding site between miR-215-3p and CXCR1 was predicted by TargetScan database.B-C. The expression of CXCR1 was detected by qRT-PCR and Western blot assays. N, normal tissues; T, tumor tissues.D. The correlation between CXCR1 and the expression of miR-215-3p and PCED1B-AS1 was analyzed by Spearman’s correlation analysis.E. The targeting relationship between CXCR1 and miR-215-3p was confirmed by dual-luciferase reporter assay.F. Western blot was used to detect the expression of CXCR1 protein in HGC-27 and NCI-N87 cells transfected with miR-215-3p mimics or inhibitors. **P < 0.01, and ***P < 0.001