Figure 5.

PCED1B-AS1 promotes the proliferation, migration, invasion and EMT of GC cells by regulating the miR-491-5p/CXCR1 axis

A-B. MiR-215-3p mimic or si-CXCR1 was co-transfected with pc-PCED1B-AS1 into NCI-N87 cells, and the expression of miR-215-3p and CXCR1 detected by qRT-PCR.C-D. CCK-8 and EdU assays were used to detect the proliferation of GC cells after transfection.E-F. Transwell assay was used to detect the migration and invasion of GC cells after transfection.G. Western blot assay was used to detect the protein levels of E-cadherin, N-cadherin, and Vimentin in GC cells after transfection.*P < 0.05, ** P < 0.01, and *** P < 0.001.