Figure 4.

miR-589-5p was sponged by circ-UBAP2. (a) The binding sequences of miR-589-5p and circ-UBAP2. (b) Luciferase activities of hRMECs. (c) The expression level of miR-589-5p when the hRMECs were transfected with si-circ-UBAP2. (d) The expression levels of miR-589-5p in the vitreous humor samples from the DR patients (n = 21) and the non-DR people (n = 21). (e) The expression levels of miR-589-5p in hRMECs treated with NG or HG. (f) FISH was performed to detect the co-location between circ-UBAP2 and miR-589-5p. Each experiment was performed in triplicate. ***P < 0.001. WT, wild type; MUT, mutant type; DR, diabetic retinopathy; si-, small interference RNA; hRMECs, human retinal microvascular endothelial cells; HG, high glucose; NG, normal glucose