Figure 5.

Inhibition of miR-589-5p abrogated the effects of si-circ-UBAP2 on oxidative stress and dysfunctions of HG-treated hRMECs. (a) The transfection efficiency of miR-589-5p. (b) MDA contents, (c) SOD, and (d) GSH-PX activities in the hRMECs were detected using the corresponding kits after the indicated treatments. (e) Western blotting was used to determine the protein expressions of Nrf2, HO-1, and SOD-1. GAPDH was used to normalize the protein expressions. (f) MTT assay was performed to determine the cell viability. (g) The migrated cells were fixed and determined using a transwell migration assay. (h) Quantification of G. (i) Images of tube-like structures were observed under an inverted microscope. (j) Quantification of I. Each experiment was performed in triplicate. *P < 0.05; **P < 0.01; ***P < 0.001. HG, high glucose; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; hRMECs, human retinal microvascular endothelial cells; MDA, malondialdehyde; SOD, superoxide dismutase; GSH-PX, glutathione peroxidase