Figure 6.

EGR1 was the target gene of miR-589-5p. (a) The binding sequences of miR-589-5p and EGR1. (b) Luciferase activities of hRMECs. (c) The transfection efficiency of EGR1. (d) The expressions of EGR1 in the vitreous humor samples from the DR patients (n = 21) and the non-DR people (n = 21). (e) The expressions of EGR1 in hRMECs treated with NG or HG. Each experiment was performed in triplicate. ***P < 0.001. WT, wild type; MUT, mutant type; DR, diabetic retinopathy; EGR1, Early Growth Response Protein 1; hRMECs, human retinal microvascular endothelial cells; HG, high glucose; NG, normal glucose