Figure 2.

Knockdown of NEAT1 enhances cell viability, and restrains cell apoptosis and inflammation. (a) Si-NEAT1 was successfully synthesized and transfected into the cells. (b) Cell viability and (c and d) apoptosis of the control group and the LPS-evoked cells transfected with si-NEAT1. (e) The protein expressions of bax, cleaved caspase-3 and bcl-2. (f) mRNA expressions of IL-1β, IL-6, IL-10, and TNF-α in the control group and the LPS-evoked cells transfected with si-NEAT1. Three independent experiments were carried out. **P < 0.01, vs. control or LPS+si-nc. LPS, lipopolysaccharide; si, small interference RNA; NEAT1, nuclear enriched abundant transcript 1; nc, negative control