Figure 3.

NEAT1 directly targets miR-211-5p. (a) Wild and mutant type of NEAT1 luciferase reporters were constructed and co-transfected with miR-211-5p into the cells. (b) Luciferase activities of the wild and mutant groups. (c) RT-qPCR was used to detect the enrichment of NEAT1 in miR-211-5p biotin-labeled group and control group. (d) MiR-211-5p expression level of the cells transfected with si-NEAT1. (e) MiR-211-5p levels of the cells incubated with different concentrations of LPS were detected. Three independent experiments were carried out. **P < 0.01, vs. mimic NC, control probe, si-nc or 0 μg/ml LPS. LPS, lipopolysaccharide; si, small interference RNA; NEAT1, nuclear enriched abundant transcript 1; wt, wild type; mut, mutant type; nc, negative control