Figure 4.

Inhibition of miR-211-5p antagonizes the effects of NEAT1 knockdown on cell viability, apoptosis and inflammation. (a) MiR-211-5p inhibitor was successfully constructed and transfected into the cells. (b) Cell viability and (c and d) apoptosis of the control group and the LPS-evoked cells co-transfected with si-NEAT1 and miR-211-5p inhibitor. (e) The protein expressions of bax, cleaved caspase-3 and bcl-2. (f) mRNA expressions of IL-1β, IL-6, IL-10, and TNF-α in the control group and the LPS-evoked cells co-transfected with si-NEAT1 and miR-211-5p inhibitor. Three independent experiments were carried out. *P < 0.05, vs. control; **P < 0.01, vs. control; ^#P < 0.05, vs. LPS+si-nc; ^&P < 0.05, vs. LPS+si-NEAT1+ inh-nc. LPS, lipopolysaccharide; si, small interference RNA; NEAT1, nuclear enriched abundant transcript 1; wt, wild type; mut, mutant type; nc, negative control