Figure 5.

MAPK1 is the downstream target gene of miR-211-5p. (a) Wild and mutant type of MAPK1 luciferase reporters were constructed and co-transfected with miR-211-5p into the cells. (b) Luciferase activities of the wild and mutant groups. (c) RT-qPCR was used to detect the enrichment of MAPK1 in miR-211-5p biotin-labeled group and control group. (d) MRNA expression levels of MAPK1 were analyzed in the miR-211-5p or miR-211-5p inhibitor transfected group. (e) MAPK1 levels of the cells incubated with different concentrations of LPS were detected. Three independent experiments were carried out. **P < 0.01, vs. miR-nc or biotin-nc; ^#P < 0.05, vs. inhibitor-nc; ^##P < 0.01, vs. inhibitor-nc. MAPK1, mitogen-activated protein kinase 1; wt, wild type; mut, mutant type; nc, negative control