Figure 6.

Overexpression of MAPK1 antagonizes the effects of NEAT1 knockdown. (a) MAPK1 overexpression vector was successfully constructed and transfected into the cells. (b) Cell viability and (c and d) apoptosis of the control group and the LPS-evoked cells co-transfected with si-NEAT1 and MAPK1. (e) The protein expressions of bax, cleaved caspase-3 and bcl-2 in the cells co-transfected with si-NEAT1 and MAPK1. (f) mRNA expressions of IL-1β, IL-6, IL-10, and TNF-α in the control group and the LPS-evoked cells co-transfected with si-NEAT1 and MAPK1. Three independent experiments were carried out. *P < 0.05, vs. control; **P < 0.01, vs. control; ^#P < 0.05, vs. LPS+si-nc; ^&P < 0.05, vs. LPS+si-NEAT1+ inh-nc. LPS, lipopolysaccharide; si, small interference RNA; NEAT1, nuclear enriched abundant transcript 1; wt, wild type; mut, mutant type; nc, negative control