Figure 5.

miR-378a-5p regulated migration and invasion ability via YEATS2 in Detroit562 cells. (a) Dual-Luciferase reporter assay was performed to detect the binding between miR-378a-5p and YEATS2 3ʹ-UTR in Detroit562 cells. (b) Expression of miR-378a-5p in obtained clinical HNSCC samples. Data were represented as mean ± SD and analyzed by paired t test (n = 17). *p < 0.05 vs. Control tissue group. (c) Cell viability was detected by CCK8 assay in Detroit562 cells after miR-378a-5p inhibitor and YEATS2 siRNA co-transfection. (d) and (e) Wound healing assay was conducted to detect the migration ability in Detroit562 cells after miR-378a-5p inhibitor and YEATS2 siRNA co-transfection (at 100 × magnification). (f) and (g) Transwell assay was conducted to detect the invasion ability in Detroit562 cells after miR-378a-5p inhibitor and YEATS2 siRNA co-transfection (at 200 × magnification). Data were represented as mean ± SD at least three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison test. *p < 0.05 vs. the indicated group