Figure 1.

DNA methylation of PRKCA in mitral valve of RHD. (a) the overview of CpG loci distribution within human PRKCA gene. (b) DNA methylation on 5ʹ terminal of PRKCA (promoter, first exon and first intron) in mitral valve of RHD by MeDIP-qPCR assay. (c) PRKCA transcription levels in mitral valve of RHD by qPCR assay. (d) PRKCA protein levels in mitral valve of RHD by WB assay. (e) PRKCA protein levels calculated by gray intensity analysis. (f) the correlation between DNA methylation and PRKCA transcription by pearson correlation analysis. the given data was processed as mean ± standard error and compared between RHD and HC groups by student’s t-test. ‘RHD’: rheumatic heart disease; ‘HC’: healthy control; ‘FC’: fold change