Figure 4.

The role of PRKCA-AS1 in regulating PRKCA expression in RHD. (a) the RNA levels of PRKCA-AS1 in TNF-α-induced AC16 and RL-14 cells. (b) DNA methylation and (c) DNMT1 occupancy on 5ʹ terminal of PRKCA (promoter, first exon and first intron) in TNF-α-induced AC16 and RL-14 cells. (d) DNMT1 occupancy and (e) DNA methylation on 5ʹ terminal of PRKCA (promoter, first exon and first intron) in TNF-α-induced AC16 and RL-14 cells treated with different truncations of exogenous PRKCA-AS1. (f) interaction between DNMT1 and different fragments of PRKCA-AS1 by RIP-qPCR assay. the given data from triplicate experiments was processed as mean ± standard error and compared by student’s t-test. ‘FC’: fold change; ‘Δ’: deletion