Figure 3.

CircCRKL suppresses AML cell proliferation by directly interacting with miR-196a-5p and miR-196b-5p. (a) the RIP assay of circCRKL. (b) the circRIP assay was performed using circFGFR1 and NC probes. (c) A schematic showing the predicted circCRKL and miR-196a-5p/miR-196b-5p binding sites, as well as the mutation of the potential miRNAs-binding sequences in circCRKL. the luciferase activity in the AML cells was co-transfected with a luciferase reporter containing either circCRKL-wt or circCRKL-mut and miR-196a-5p/miR-196b-5p mimics. (d) MiR-196a-5p and miR-196b-5p expression in the AML samples. (e) the correlation between the miR-196a-5p/miR-196b-5p expression and circCRKL levels. (f) the CCK-8 assay of the indicated AML cells. (g) the colony formation assay of the indicated cells. **P < 0.01, ***P < 0.001