Figure 4.

P27 is regulated by miR-196a-5p and miR-196b-5p and inhibits cell proliferation. (a) the p27-3’ UTR sequences containing miR-196a-5p and miR-196b-5p binding sites. (b) the luciferase activity in the AML cells co-transfected with a luciferase reporter containing either p27 3’ UTR-wt or p27 3’ UTR-mut and miR-196a-5p/miR-196b-5p mimics. (c) the p27 expression levels in the THP1 and MOLM13 cells transfected with miR-196a-5p/miR-196b-5p mimics alone or co-transfected with circCRKL or sh-circCRKL. (d) the p27 protein expression in the AML cells. (e) the CCK-8 assay of AML cells. (f, g) the cell cycle assay of the AML cells. (h, i) the colony formation assay of the AML cells. *P < 0.05, **P < 0.01, ***P < 0.001