Figure 4.

Inhibition of micro RNA miR-122-5p suppresses lipopolysaccharide (LPS)-induced oxidative stress and inflammatory response. (a) Reactive oxygen species (ROS) production was determined by a flow cytometer. (b) The enzymes catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-px) activities were measured using the manufacturer’s kits. (c) Western blot was performed to measure the nuclear factor erythroid 2-related factor 2 (Nrf-2) expression in the cytoplasm and nucleus. (d) Real-time quantitative PCR (RT-qPCR) was used for heme oxygenase-1 (HO-1) and NAD(p)H: quinone oxidoreductase 1 (NQO-1) levels. (e) The concentrations of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6) and IL-1β were quantified by enzyme-linked immunosorbent assay (ELISA) kits. ***p < 0.001 versus control; ⁺p < 0.05, ⁺⁺p < 0.01, ⁺⁺⁺p < 0.001 versus LPS + NC inhibitor