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. 2021 Jul 19;12(1):3566–3577. doi: 10.1080/21655979.2021.1947939

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — The relationship between SP1 and STK39

(a,b) The expression correlation and binding sequences between SP1 and STK39 was analyzed using GEPIA database. (c) The result of dual-luciferase report assay. ***P < 0.001 vs STK39-WT + pcDNA3.1. (d) Chromatin immunoprecipitation (ChIP) assay was adopted to detect the interaction between STK39 and SP1. ***P < 0.001 vs IgG. (e-f) The transfection efficiency of SP1 overexpression in Hep3b cells. **P < 0.01 and ***P < 0.001 vs Control; ^##P < 0.01 and ^###P < 0.001 vs pcDNA3.1. (g,h) The mRNA and protein expression of STK39 in Hep3b cells that transfected with indicated vectors was examined with RT-qPCR and western blot assay. ***P < 0.001 vs Control; ^###P < 0.001 vs shRNA-NC; ^$$$P < 0.001 vs shRNA-STK39; ^&&&P < 0.001 vs shRNA-STK39+ pcDNA3.1.