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. 2021 Oct 21;12(1):8173–8185. doi: 10.1080/21655979.2021.1977766

Figure 3.

Figure 3.

MiR-15a-5p targets 3ʹUTR of PDCD4 and regulate osteogenic differentiation

a: Binding site of mutant and wild type 3ʹUTR of PDCD4 compared to the miR-15a-5p. b: Relative luciferase activity to represent the binding of miR-15a-5p and 3ʹUTR of PDCD4. C: Relative mRNA expression of PDCD4 in MC3T3-E1 cells after transfection of miR-15-a-5p mimics and inhibitors. d-e: Relative protein expression of PDCD4 in MC3T3-E1 cells after transfection of miR-15-a-5p mimics and inhibitors. f: Relative PDCD4 mRNA expression in MC3T3-E1 induced with osteogenic media. g: Relative PDCD4 mRNA expression in MC3T3-E1 cells after transfection of si-PDCD4. h: Relative mRNA expression of ALP, OCN and RUNX2 genes detected in MC3T3-E1 cells after transfection of si-PDCD4. I: Western blot showing expression of PDCD4, ALP, OCN and RUNX2 proteins in MC3T3-E1 cells after transfection of si-PDCD4. j: Relative ALP activity in MC3T3-E1 cells after transfection of si-PDCD4. k: Alizarin Red staining of MC3T3-E1 cells after transfection of si-PDCD4. (Data presented as means ± SEM; **p < 0.01)