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. 2021 Sep 1;12(1):5373–5385. doi: 10.1080/21655979.2021.1960462

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Characterization of circNfix in cardiomyocytes. (a) CircNfix was back-spliced by exons 2 of Nfix gene as the black arrow showed. The red arrow indicates head-to-tail splicing junction site of circNfix. (b) The expressions of linear mRNA Nfix and circNfix in RNase R-treated cardiomyocytes were detected with RT-qPCR. (c) RT-qPCR analysis of circNfix and Nfix mRNA in actinomycin D-treated cardiomyocytes. (d) CircNfix has been validated using nucleic acid electrophoresis. (e) The distribution of circNfix in cardiomyocytes was examined by RT-qPCR. (f, g) RT-qPCR was used to detect the expression of circNfix in TAC-treated mice and in Ang II treated cardiomyocytes. **P < 0.01, compared with control group; n = 3