Figure 4.

Analysis of the direct target gene of miR-148a-3p. A: Differential analysis of mRNAs with red for up-regulated mRNAs and green for down-regulated mRNAs; B: Analyzed miR-148a-3p potential target genes; C: Screened the target genes that negatively regulated by miR-148a-3p. Red for up-regulation, green for down-regulation, triangle for miRNA, and circle for mRNA; D: wt CDK6 or mt CDK6 reporter plasmid and miR-NC or miR-148a-3p mimics were co-transfected with J111 and KG-1a cells for 48 h, and DLR experiment was conducted to measure the luciferase activity; E: miR-148a-3p mimics or miR-NC was co-transfected with J111 and KG-1a cells for 48 h, qRT-PCR and Western blot measured CDK6 mRNA and protein expressions; F: miR-148a-3p inhibitor or inhibitor-NC was transfected into J111 and KG-1a cells for 48 h, and CDK6 mRNA and protein expressions were detected by qRT-PCR and Western blot. Compared with miR-NC, **P < 0.01