LINC00958 promoted cell growth, but inhibited cell apoptosis of colorectal cancer cells
(a) Measurement of LINC00958 expression in colorectal cancer cell lines (SW620, HCT116 and HCT8) and normal colorectal epithelial cells FHC. (b) RT-qPCR analysis of LINC00958 in colorectal cancer tissues and normal tissues. (c) The existence of GAPDH, LINC00958, and U6 in the cytoplasm and nucleus were detected in HCT116 and HCT8 cell lines by RT-qPCR. (d) RT-qPCR analysis of LINC00958 in HCT116 and HCT8 cells transfected with NC and si-LINC00958. (e) Cell viability was detected in HCT116 and HCT8 cells transfected with NC and si-LINC00958 by CCK8 assay. (f) Cell proliferation was detected in HCT116 and HCT8 cells transfected with NC and si-LINC00958 by EdU assay. (g) Cell apoptosis was determined in HCT116 and HCT8 cells transfected with NC and si-LINC00958 by FITC apoptosis detection kit. *, P < 0.05; **, P < 0.001 compared with blank. NC, negative control; si-LINC00958, siRNA-LINC00958.