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. 2021 Oct 21;12(1):8100–8115. doi: 10.1080/21655979.2021.1985259

Figure 7.

Figure 7.

MiR-3064-5p targeted to LEMD1 and inhibited the expression of LEMD1 in colorectal cancer cells

(a) StarBase showed the predicted binding sequences of LEMD1 and miR-3064-5p. (b) Dual luciferase assay was performed in cells co-transfected with plasmids LEMD1 3ʹUTR-WT or LEMD1 3ʹUTR-MUT and NC mimics or miR-3064-5p mimic in HCT116 and HCT8 cells. (c) RNA pull-down assay was performed between miR-3064-5p and LEMD1 in HCT116 and HCT8 cells. (d) The correlation between the relative expression level of miR-3064-5p and LEMD1 in colorectal cancer tissues. (e) RT-qPCR detection of LEMD1 expression in colorectal cancer cells (HCT116 and HCT8 cells) and normal FHC cells. (f) Measurement of LEMD1 protein expression in HCT116 and HCT8 cells transfected with NC, Si-LEMD1, miR-3064-5p inhibitor, and Si-LEMD1+ miR-3064-5p inhibitor. *, P < 0.05; **, P < 0.001 compared with blank. #, P < 0.05; ##, P < 0.001 compared with Si-LEMD1+ inhibitor. NC, negative control; WT, wild-type; MUT, Mutant; Si-LEMD1, SiRNA-LEMD1; Si-LEMD1+ miR-3064-5p inhibitor, SiRNA-LEMD1+ miR-3064-5p inhibitor.