Figure 2.

Downregulation of SATB1-AS1 mitigates the chemoresistance of AML cells in vitro. (a) The IC50 values of parental cells overexpressing SATB1-AS1 and of HL60/Adr and OCI-AML5/Cyt cells harboring silencing SATB1-AS1 measured by CellTiter-Glo assay. Adr (5 μM) was used to treat HL60 parental cells and resistant cells and 10 μM Cyt to treat OCI-AML5 parental cells and resistant cells, respectively. (b) Cell apoptosis tested by flow cytometry. (c) Hoechst 33,258 staining for cell apoptosis. (d) Cell proliferation evaluated by colony formation assay. (e) EdU staining for cell viability. (f) AML cell cytotoxicity evaluated by an LDH kit. Each assessment was done in triplicate with 3-time repetition to ensure minimum deviation; statistical data were measurement data, and described as mean ± standard deviation; one-way (panels b–f) or two-way ANOVA (panel a) was applied for multiple-group comparisons, followed by Tukey’s multiple comparisons test. * p < 0.05