Figure 6.

Inactivation of PPAR-γ signaling reversed the effects of FMNT on ox-LDL-induced endothelial injury. (a) Western blot assay was applied to evaluate the effect of FMNT on PPAR-γ expression in ox-LDL-treated HUVECs. (b-g) PPAR-γ antagonist (GW9662, 5 μM) was used to block PPAR-γ signaling in HUVECs prior to ox-LDL and FMNT treatment, followed by assays of PPAR-γ protein expression (b), cell viability (c), TNF-α and IL-1β level in culture supernatant (d and e), intracellular ROS level (f) and apoptotic rate (g). ***P < 0.001