Figure 1.

Circ0005654 is elevated in GC tissues and cells, which predicts a dismal survival. A, prediction of circ0005654 location, length, and host gene by circBase and UCSC websites; B, expression of circ0005654 in GC and adjacent tissues by RT-qPCR (*p < 0.05 by the paired t test); C, evaluation of the diagnostic effect of circ0005654 by a ROC curve using the Wilson/Brown analysis); D, survival analysis of patients with high or low expression of circ0005654 using the Kaplan-Meier analysis); E, circ0005654 expression in GC cells and GES-1 cells by RT-qPCR (*p < 0.05, **p < 0.01 by one-way ANOVA); F, the cellular localization of circ0005654 in MKN-45 and HGC-27 cells determined by FISH. Values are displayed as the mean ± SD on the basis of three independent experiments