Figure 7.

Sp1 binds to myc, thereby mediating the Wnt/β-catenin pathway. A, prediction of downstream genes and transcriptional sites of sp1 by bioinformatics analysis; B, the binding relation between myc and sp1 verified by ChIP experiments (*p < 0.05 by the two way ANOVA); C, myc expression between GC and adjacent tissues examined using RT-qPCR (*p < 0.05 by the paired t test); D, Person’s correlation analysis of sp1 and myc expression in GC tissues; E, expression of myc in MKN-45 cells after transfection examined using RT-qPCR (*#p < 0.05 by the one way ANOVA); F, the protein expression of myc and β-catenin in MKN-45 cells examined using western blot (*#p < 0.05 according to the two way ANOVA); G, the protein expression of sp1, myc and β-catenin in MKN-45 cells after circ0005654 knockdown examined using western blot (*#p < 0.05 according to the two way ANOVA); H, the protein expression of myc and β-catenin in xenografted mouse tumor tissues examined using western blot (*#p < 0.05 according to the two way ANOVA)