Figure 4.

Down-regulation of miR-106b-5p reversed the effects of H19 knockdown on cell viability and ferroptosis of BMVECs

(a) miR-106b-5p expression was detected using RT-qPCR after H19`knockdown. (b) Cell viability was detected using CCK8 assays after transfection with si-H19. (c) PI staining was used to detect cell death. (d) TUNEL staining was used to detect cell death. (E-H) Levels of iron, GSH, MDA, and LPO. (i–k) Levels of SLC7A11, GPX4, and TFR1 mRNAs were measured using RT-qPCT. (l) Expression of SLC7A11, GPX4, and TFR1 proteins detected by western blotting. **P < 0.01, compared with NC inhibitor group or control group. #P < 0.05, ##P < 0.01, compared with OGD/H + si-lnc-H19 + NC inhibitor group.