Figure 6.

Up-regulation of ACSL4 inhibited the effects of miR-106b-5p

(a) ACSL4 expression was detected using RT-qPCR after miR-106b-5p overexpression. (b) Cell viability was detected using CCK8 assays after transfection with miR-106b-5p mimic. (c) PI staining was used to detect cell death. (d) TUNEL staining was used to detect cell death. (e–h) Levels of iron, GSH, MDA, and LPO. (i–k) Expression of SLC7A11, GPX4, and TFR1 mRNAs were measured using RT-qPCT. (l) Expression of SLC7A11, GPX4, and TFR1 proteins detected by western blotting.**P< 0.01, compared with vector group or control group. #P < 0.05, ##P < 0.01, compared with the OGD/H + mimic + vector group.