Figure 3.

Targeting of HOXB1 by miR-301b-3p in CRC. (a) 3ʹ-UTR region of HOXB1 with the binding site for miR-301b-3p in WT and mut plasmids. The luciferase activity of HOXB1 WT or MUT 3ʹ-UTR with miR-301b-3p mimics in CRC. (b,c) The mRNA and protein level of HOXB1 affected with miR-301b-3p mimics in HT-29 and DLD-1. (d) The expression of HOXB1 mRNA is unregulated in adjacent of colorectal tissues. (e) The relationship of HOXB1 and miR-301b-3p in CRC. (R² = −0.55, P< 0.01). *P < 0.05 and **P < 0.01