Figure 4.

MiR-4537 targets ZNF587 and negatively regulates ZNF587 expression

(a) The expression of ZNF587 in GC cells was shown by RT-PCR at different points of time after radiation. (b) RT-PCR was used to detect the effect of miR-4537 mimics on the expression level of ZNF587. (c) Western blot was used to examine the effect of miR-4537 mimics on the protein level of ZNF587. (d) Binding sites between miR-4537 and ZNF587 3ʹUTR was shown. (e) The luciferase reporter assay was performed to verify the relationship between miR-4537 and ZNF587. *p< 0.05, ** p < 0.01, *** p< 0.001.