Figure 3.

miR-136-5p was negatively regulated by circ_RANBP9. (a) Direct binding sites of miR-136-5p in WT 3ʹ-UTR of circ_RANBP9 and MUT 3ʹ-UTR of circ_RANBP9 were designed. (b) WT circ_RANBP9 or MUT circ_RANBP9 recombinant plasmids and miR-136-5p mimic or mimic nc were co-transfected and luciferase reporter assay was performed. (c) miR-136-5p levels determined using RT-qPCR were detected in GC overexpression or knockdown of circ_RANBP9. (d) Interaction between miR-136-5p and circ_RANBP9 was confirmed by RNA pull-down. (e) Expression of miR-136-5p in normal ovarian epithelium (IOSE80) cells and GCs (KGN, COV434) was detected by RT-qPCR. **P < 0.01. ^##P < 0.01