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. 2021 Sep 21;12(1):6748–6758. doi: 10.1080/21655979.2021.1964157

Figure 4.

Figure 4.

Inhibition of miR-136-5p abolished the effects on proliferation and apoptosis induced by knockdown of circ_RANBP9. (a) Expression of miR-136-5p was tested in transfected cells by RT-qPCR. Cell proliferation was assessed post-transfection by (b) MTT assay and (c) colony formation assay. (d) Flow cytometry was performed to detect apoptosis rates of GCs. (e) Protein levels of caspase-3, Bax, and Bcl-2 at was determined by western blotting. **P < 0.01. ##P < 0.01