Figure 1.

CircPUM1 knockdown induces dysfunction and inflammation in HTR-8/SVneo cells. (a) CircPUM1 expression was determined by RT-qPCR in placental tissues of RSA patients and normal controls. (b) CircPUM1 expression was determined in HTR-8/SVneo cells after transfection with si-circPUM1 and si-NC. (c) CCK-8 analysis revealed a significant reduction in cell viability following circPUM1 knockdown. (d and e) Transwell assays showed that circPUM1 depletion inhibited cell migration and invasion. (f) Flow cytometry revealed an increase in apoptosis following circPUM1 knockdown. (g) The cleaved caspase-3 level was detected by Western blotting. (h-j) CircPUM1 knockdown promoted TNF-α (h), IL-6 (i), and IL-8 (j) expressions, as determined by ELISA. All data were obtained from three independent experiments (n = 3). *P < 0.05 and **P < 0.01