Figure 2.

CircPUM1 sponges miR-30a-5p in HTR-8/SVneo cells. (a) The expression of miRNAs was appraised in cells transfected with si-circPUM1 and si-NC. (b) The binding site between miR-30a-5p and circPUM1 was predicted by StarBase website. (c) The expression of miR-30a-5p was examined in cells transfected with miR-30a-5p mimics and NC mimics. (d and e) Luciferase reporter and RIP assays were conducted to assess the binding of miR-30a-5p and circPUM1 in HTR-8/SVneo cells. (f) miR-30a-5p expression was tested in placental tissues of RSA patients and normal controls. (g) Pearson’s correlation analysis was used to analyze the association between miR-30a-5p and circPUM1 expressions in RSA placental tissues. All data were obtained from three independent experiments (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001